Sitakanta Pattanaik

Sitakanta Pattanaik

Sitakanta Pattanaik

Sitakanta Pattanaik

Principal Investigator, Scientist III

University of Kentucky 1401 University Drive Lexington, KY 40546-0236

Last Revised: Feb 8th, 2024

Professional Biography

I have been working at KTRDC since 2001:

2001 - 2004          post-doctoral scientist with Dr. Indu B. Maiti

2004 - 2005          post-doctoral scientist with Dr. Ling Yuan

2005 - 2009          Scientist I with Dr. Ling Yuan

2009 - 2018          Scientist II

2018 - present      Scientist III

Research Interests

1. Transcriptional and posttranscriptional regulation of secondary metabolism in plants

2. Isolation and characterization of novel promoters for plant genetic engineering

Selected publications

Lim ARQ, Kong Q, Noor A, Song YQ, Pattanaik S, Yuan L, Ma W (2023) B-BOX-DOMAIN PROTEIN32 modulates seed oil biosynthesis in Arabidopsis by interacting with WRINKLED1. Plant Physiology 193:2919–922.

Hou X, Singh SK, Werkman JR, Liu Y, Yuan Q, Wu X, Patra B, Sui X, Lyu R, Wang B, Liu X, Li Y, Ma W, Pattanaik S, Yuan L (2023) Partial desensitization of MYC2 transcription factor alters the interaction with jasmonate signaling components and affects specialized metabolism. International Journal of Biological Macromolecules. 252:126472

Liu Y, Singh SK, Pattanaik S, Wang H, Yuan L (2023) Light regulation of the biosynthesis of phenolics, terpenoids, and alkaloids in plants. Communications Biology. 6(1):1055.

Singh SK, Patra B, Singleton JJ, Liu Y, Paul P, Sui X, Suttipanta N, Pattanaik S, Yuan L (2022) Identification and Characterization of Transcription Factors Regulating Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseusMethods in Molecular Biology. 2505:203-221.

Patra B, Liu Y, Singleton JJ, Singh SK, Pattanaik S, Yuan L. (2022) Virus-induced gene silencing as a tool to study regulation of alkaloid biosynthesis in medicinal plants. Methods in Molecular Biology. 2469:155-164.

Wu Z, Singh SK, Lyu R, Pattanaik S, Wang Y, Li Y,Yuan L and Liu Y (2022) Metabolic engineering to enhance the accumulation of bioactive flavonoids licochalcone A and echitanin in Glycyrrhiza inflata (licorice) hairy roots. Frontiers in Plant Science 13:932594.

Liu YL, Patra B, Singh SK, Paul P, Zhou Y Li YQ, Wang Y, Pattanaik S, Yuan L. (2021) Terpenoid indole alkaloid biosynthesis in Catharanthus roseus: effects and prospects of environmental factors in metabolic engineering. Biotechnology Letters 43: 2085-2103.

Lyu R, Singh SK, Liu Y, Patra B, Zhou Y, Wang B, Pattanaik S, Yuan L. (2021) Reprogramming plant specialized metabolism by manipulating protein kinases. aBiotech 2:226-239.

Singh SK, Patra B, Paul P, Pattanaik S, Yuan L. (2021) BHLH IRIDOID SYNTHESIS 3 is a member of a bHLH gene cluster regulating terpenoid indole alkaloid biosynthesis in Catharanthus roseusPlant Direct 00:e00305. 

Liu X, Singh SK, Patra B, Liu Y, Wang B, Wang J, Pattanaik S, Yuan L. (2020) Protein-phosphatase NtPP2C2b and MAP-kinase NtMPK4 act in concert to modulate nicotine biosynthesis. Journal of Experimental Botany. 72(5):1661-1676.

Singh SK, Richmond MD, Pearce RC, Bailey WA, Hou X, Pattanaik S, Yuan L (2020) Maleic hydrazide elicits global transcriptomic changes in chemically topped tobacco to influence shoot bud development. Planta 252(4):64.

Kong Q, Singh SK, Mantyla JJ, Pattanaik S, Guo L, Yuan L, Benning C, Ma W (2020) TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR 4 interacts with WRINKLED1 to mediate seed oil biosynthesis. Plant Physiology 184:658-665.

Singh SK, Patra B, Paul P, Liu Y, Pattanaik S, Yuan L (2020) Revisiting the ORCA gene cluster that regulates terpenoid indole alkaloid biosynthesis in Catharanthus roseusPlant Science 293:110408.

Paul P, Singh SK, Patra B, Liu X, Pattanaik S, Yuan L. (2020) Mutually Regulated AP2/ERF Gene Clusters Modulate Biosynthesis of Specialized Metabolites in Plants. Plant Physiology 182:840-856.

Liu YL, Patra B., Pattanaik S, Wang Y, Yuan L. (2019) GATA and PIF transcription factors regulate light-induced biosynthesis of vindoline in Catharanthus. Plant Physiology 180:1336-1350. 

Sui X, Kumar Singh S, Patra B, Schluttenhofer C, Guo W, Pattanaik S, Yuan L. (2018) Cross-family transcription factor interaction between MYC2 and GBFs modulates terpenoid indole alkaloid biosynthesis. Journal of Experimental Botany 69: 4267-4281.

Wang J, Wang X, Geng S, Singh SK, Wang Y, Pattanaik S, Yuan L. (2018) Genome-wide identification of hexokinase gene family in Brassica napus: structure, phylogenetic analysis, expression, and functional characterization. Planta. 248:171-182

Patra B, Pattanaik S, Schluttenhofer C, Yuan L. (2018) A network of jasmonate-responsive bHLH factors modulate monoterpenoid indole alkaloid biosynthesis in Catharanthus roseusNew Phytologist 217:1566-1581.

Paul P, Singh SK, Patra B, Sui X, Pattanaik S, and Yuan L (2017). A differentially regulated AP2/ERF transcription factor gene cluster acts downstream of a MAP kinase cascade to modulate terpenoid indole alkaloid biosynthesis in Catharanthus roseus. New Phytologist 213:1107-1123.

Shen EM, Singh SK, Ghosh JS, Patra B, Paul P, Yuan L, and Pattanaik S (2017). The miRNAome of Catharanthus roseus: identification, expression analysis, and potential roles of microRNAs in regulation of terpenoid indole alkaloid biosynthesis. Sci Rep 7:43027.

Wang H, Yang J, Zhang M, Fan W, Firon N, Pattanaik S, Yuan L and Zhang P (2016) Altered Phenylpropanoid Metabolism in the Maize Lc-Expressed Sweet Potato (Ipomoea batatas) Affects Storage Root Development. Sci Rep 6:18645

Singh SK, Wu Y, Ghosh JS, Pattanaik S, Fisher C, Wang Y, Lawson D and Yuan L (2015) RNA-sequencing Reveals Global Transcriptomic Changes in Nicotiana tabacum Responding to Topping and Treatment of Axillary-shoot Control Chemicals. Sci Rep 5:18148.

Schluttenhofer C, Pattanaik S, Patra B and Yuan L (2014) Analyses of Catharanthus roseus and Arabidopsis thaliana WRKY transcription factors reveal involvement in jasmonate signaling. BMC Genomics. 15:502.

Pattanaik S, Patra B, Singh, SK and Yuan L (2014) An overview of the gene regulatory network controlling trichome development in the model plant Arabidopsis. Front Plant Sci. 5:259.

 Patra, B, Schluttenhofer C, Pattanaik S and Yuan L (2013) Transcriptional regulation of secondary metabolite biosynthesis in plants. Biochim Biophys Acta. 1829:1236-1247.

Yang Z, Patra B, Li R, Pattanaik S and Yuan L (2013) Promoter analysis reveals cis-regulatory motifs associated with the expression of the WRKY transcription factor CrWRKY1 in Catharanthus roseus. Planta 238:1039-1049.

Patra B, Pattanaik S and Yuan L (2013) Ubiquitin protein ligase 3 mediates the proteasomal degradation of GLABROUS 3 and ENHANCER OF GLABROUS 3, regulators of trichome development and anthocyanin biosynthesis in Arabidopsis. Plant J. 74:435-447.

Kong Q, Pattanaik S, Feller A, Werkman JR, Chai C, Wang Y, Grotewold E and Yuan  L (2012) Regulatory switch enforced by basic helix-loop-helix and ACT-domain mediated dimerizations of the maize transcription factor R. Proc Natl Acad Sci USA. 109: E2091-2097

Bai Y, Pattanaik S, Patra B, Werkman JR, Xie CH, and Yuan L (2011) Flavonoid-related basic helix-loop-helix regulators, NtAn1a and NtAn1b, of tobacco have originated from two ancestors and are functionally active. Planta  234:363-375.

Suttipanta N, Pattanaik S, Kulshrestha M, Patra B, Singh SK, and Yuan L (2011) The transcription factor CrWRKY1 positively regulates terpenoid indole alkaloid biosynthesis in Catharanthus roseus. Plant Physiol. 157:2081-2093.

Pattanaik S, Kong Q, Zaitlin D, Werkman JR, Xie CH and Yuan L (2010) isolation and Functional Characterization of a floral tissue-specific R2R3 Myb regulator from tobacco. Planta 231:1061-1076.

Pattanaik S, Xie CH and Yuan L (2008) The interaction domains of the plant Myc-like bHLH transcription factors can regulate the transactivation strength. Planta 227:707-715

Pattanaik S, Nitima Suttipanta N, Gunjan S, Xie CH, Littleton J and Yuan L (2007) Promoter analysis of the Catharanthus roseus geraniol 10-hydroxylase gene involved in terpenoid indole alkaloid biosynthesis. Biochim Biophys Acta 1769:139–148.

Hou C-X , Dirk LMA, Pattanaik S, Das NC, Maiti IB, Houtz RL and Williams MA (2007) Plant peptide deformylase: a novel selectable marker and herbicide target based on essential cotranslational chloroplast protein processing. Plant Biotech J. 5: 275-281.

Pattanaik S, Xie CH, Kong Q, Shen KA, Yuan L (2006) Directed evolution of plant basic helix–loop–helix transcription factors for the improvement of transactivational properties. Biochim Biophys Acta 1759:308–318.

Pattanaik S, Dey N, Bhattacharyya S and Maiti, IB (2004) Isolation of a full-length transcript promoter from the Strawberry vein banding virus (SVBV) and expression analysis by protoplasts transient assays and in transgenic plants. Plant Sci. 167:427-438.

Majee M, Maitra S, Ghose Dastidar K, Pattnaik S, Chatterjee A, Hait N, Das KP, and Lahiri Majumder A (2004) A  novel salt-tolerant L-myo-inositol 1-phosphate synthase from Porteresia coarctata Tateoka, a halophytic wild rice: Molecular cloning, bacterial overexpression, characterization  and functional introgression  into tobacco conferring salt-tolerance phenotype. J Biol Chem. 279:28539-28552.

Bhattacharyya S, Pattanaik S and Maiti IB (2003) Intron-mediated enhancement of gene expression in transgenic plants using chimeric constructs composed of Peanut chlorotic streak virus (PClSV) promote-leader and the antisense orientation of PClSV ORF VII (p7R).  Planta  218: 115-124.

Maiti IB, Dey N, Pattanaik S, Dahlman DL, Rana RL and Webb BA (2003) Antibiosis-type insect resistance in transgenic plants expressing a teratocyte secretory protein (TSP14) gene from a hymenopteran endoparasite (Microplitis croceipes).  Plant Biotech J. 1:209-219.

Book Chapters

Singh S, Patra B, Sigleton J, Liu Y, Paul P, Sui X, Suttipanta N, Pattanaik S and Yuan L (2022) Identification and Characterization of Transcription Factors Regulating Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus. In: Catharanthus roseus: Methods and Protocols. V. Courdavault and S. Besseau (ed.), Methods Mol Biol 2505:203-221.

Patra B, Liu Y, Singleton J, Singh S Pattanaik S, and Yuan L (2022) Virus-Induced Gene Silencing as a Tool to Study Regulation of Alkaloid Biosynthesis in Medicinal Plants. In: Plant Secondary Metabolism Engineering: Methods and Protocols. A.G. Fett-Neto (ed.), Methods Mol Biol 2469:155-164.

Pattanaik S, Werkman JR, and Yuan L (2011) Bimolecular Fluorescence Complementation as a Tool to Study Interactions of Regulatory Proteins in Plant Protoplasts. In: Plant Transcription Factors. L. Yuan and S.E. Perry (eds.), Methods Mol Biol 754:185-193.

Werkman JR, Pattanaik S, and Yuan L (2011) Directed Evolution Through DNA Shuffling for the Improvement and Understanding of Genes and Promoters. In: In: Plant Transcription Factors. L. Yuan and S.E. Perry (eds.), Methods Mol Biol 754:325-342

Pattanaik S, Werkman JR, Kong Q and Yuan L (2010) Site-directed Mutagenesis and Saturation Mutagenesis for the Functional Study of Transcription Factors Involved in Plant Secondary Metabolite Biosynthesis.  In: Plant Secondary Metabolism Engineering, A. G. Fett-Neto (ed.), Methods Mol Biol 643:47-57

Maiti IB, Pattanaik S, Bhattacharyya S and Dey N (2004) Genetic tools useful for basic research in molecular biology and biotechnological applications. In: Biotechnological Approaches for Sustainable Development. M.S. Reddy and S. Khanna (eds.), Chapter 6, pages 29-39, Allied Publishers Pvt Ltd., India.

Lahiri Majumder A, Hait NC, Deb I, Majee M, Chatterjee A, Ghosh Dastidar K,  Bhattacharyya S, Ghosh S, Chatterjee A, Maitra S and Pattanaik S (2003) L-myo inositol 1-phosphate synthase: an ancient protein with diverse function.  In: Molecular Insight in Plant Biology. P. Nath, A.K. Mattoo, S.A. Ranade and J.H. Weil (eds.), Chapter 5, pages 67-76, Science Publishers, USA.

Pattnaik S and Chand PK (1996) "Artificial seeds" as an aid to clone White Mulberry (Morus alba L.). In: Poncelet D & Dautzenberg H (eds.) Proceedings International Workshop Bioencapsulation V: From Fundamentals to Industrial Application.  Potsdam, Germany, pp.165-68.


Ph.D., Plant Science, Utkal University

Contact Information

1401 University Drive Lexington, KY 40546